| 摘要 | 第1-7页 |
| Abstract | 第7-10页 |
| List of Figure and Table | 第10-11页 |
| 1. Introduction | 第11-14页 |
| 2. Materials and method | 第14-19页 |
| ·Chemicals, materials and Apparatus | 第14-15页 |
| ·Preparation of plant sample | 第15页 |
| ·Two dimensional pre-column based on short carbon fibers system | 第15-18页 |
| ·Fabrication of CFs column and ACFs column | 第15页 |
| ·Processing of two dimensional pre-column system | 第15-18页 |
| ·Instrumental Analysis | 第18-19页 |
| 3. Results and Discussion | 第19-29页 |
| ·The choice of various polar targets | 第19-20页 |
| ·Characterization of carbon fibers and activated carbon fibers | 第20-21页 |
| ·Optimization of size of carbon fibers column and activated carbon fibers column | 第21-22页 |
| ·Optimization of elution solvent kinds and volume | 第22-23页 |
| ·Optimization of flow rate of elution solvent | 第23页 |
| ·Two dimensional separation on the CFs column and ACFs column | 第23-24页 |
| ·Compare separation results of standards mixture with and without CFs column by HPLC-UV | 第24-25页 |
| ·Performance of the method | 第25-27页 |
| ·Applied two dimensional pre-separation based on short carbon fibers column to real Chinese medicine sample | 第27-29页 |
| 4. Conclusion | 第29-30页 |
| 致谢 | 第30-31页 |
| References | 第31-38页 |
| 附录A | 第38-39页 |
