| ACKNOWLEDGEMENT | 第1-14页 |
| <英文摘要> | 第14-15页 |
| <英文关键词> | 第15-16页 |
| <中文摘要> | 第16页 |
| <中文关键词> | 第16-17页 |
| Chapter 1 GENERAL INTRODUCTION AND OBJECTIVE OF STUDIES | 第17-20页 |
| ·INFECTIOUS BRONCHITIS | 第17-18页 |
| ·Proventriculitis | 第18-19页 |
| ·Objectives of The Present Studies | 第19-20页 |
| Chapter 2 LITERATURE REVIEW | 第20-28页 |
| ·INFECTIOUS BRONCHITIS VIRUS | 第20-21页 |
| ·The morphology of IBV | 第21-22页 |
| ·Genome organization of IBV | 第22-23页 |
| ·Structural characteristics of S glycoprotein | 第23-24页 |
| ·Antigenic topography of IBV spike protein | 第24页 |
| ·Functional characteristics of S1 glycoprotein | 第24-25页 |
| ·Evidence of recombination linked to spike of S1 glycoprotein | 第25页 |
| ·Proventriculitis | 第25-28页 |
| Chapter 3 ISOLATION OF FIELD ISOLATES OF PROVENTRICULOPATHOGENIC INFECTIOUS BRONCHITIS VIRUS | 第28-35页 |
| Materials | 第28页 |
| Methods | 第28页 |
| ·Adaption of the pathogens in chicken embryos | 第28-29页 |
| ·Virus propagation in embryonated eggs | 第29页 |
| ·Collection of specimens from embronating eggs | 第29页 |
| ·Morphology of 2/97,3/97 and 1/98 isolates | 第29页 |
| ·Physical and Chemical properites | 第29-30页 |
| ·Biological characteristics of the virus isolates | 第30-31页 |
| ·Serological identification | 第31页 |
| Results | 第31-35页 |
| Chapter 4 CLONING AND SEQUENCE ANALSIS OF S1 GENE IBV ISOLATES 2/97, 3/97 AND 1/98 | 第35-66页 |
| Materials | 第35页 |
| Methods | 第35页 |
| ·Virus propagation and purification | 第35页 |
| ·Reverse Transcriptase polymerase reaction | 第35-37页 |
| ·Cloning and identification of PCR products | 第37-40页 |
| ·Sequencing and sequence analysis | 第40-41页 |
| Results | 第41-44页 |
| ·Determination of nucleotide sequence of S1 gene from the virus isolates 2/97,3/97 and 1/98 | 第44-49页 |
| ·Comparisou of S1 gene of several IBV strains | 第49-58页 |
| ·Comparison of the AA sequeuces of several IBV S1 subunits | 第58-61页 |
| ·Analysis of tbe hydrophobic index of the S1 subunit | 第61-63页 |
| ·The phylogenetic tree of several IBV strains according to S1 gene | 第63-66页 |
| Chapter 5 LABORATORY TRIAL OF INACTIVATED OIL EMULSION VACCINE AGAINST PROVENTRICULOPATHOGENIC STRAINS OF INFECTIOUS BRONCHITIS VIRUS | 第66-72页 |
| Materials | 第66页 |
| Methods | 第66页 |
| ·preparation of Virus antigen | 第66页 |
| ·Virus inactivation | 第66-67页 |
| ·Vaccine preparation | 第67-68页 |
| Results | 第68-69页 |
| ·Vaccine efficacy | 第69-71页 |
| ·DISCUSSION ON VACCINE | 第71-72页 |
| Chapter 6 Discussion,Conclusions and Recommendations | 第72-75页 |
| ·DISCUSSION | 第72-73页 |
| ·Conclusion | 第73页 |
| ·RECOMMENDATIONS | 第73-75页 |
| <参考文献> | 第75-79页 |
