| Abstract | 第1-12页 |
| 中文摘要 | 第12-14页 |
| Chapter 1.Introduction | 第14-19页 |
| ·Gangliosides and ganglioside GD1a | 第14页 |
| ·Caveolae and caveolin-1 | 第14-15页 |
| ·Cell death and apoptosis | 第15-16页 |
| ·MAPKs and PI3K pathway | 第16-17页 |
| ·Cellular endocytosis | 第17页 |
| ·Summary | 第17-19页 |
| Chapter 2.Meterials and methods | 第19-26页 |
| ·Cell lines and cell culture | 第19页 |
| ·Chemicals | 第19页 |
| ·Glycolipid extraction and high performace thin layer chromatography(HPTLC) | 第19-20页 |
| ·Small interference RNA(siRNA)and siRNA transfection | 第20-21页 |
| ·SiRNA sequence | 第20页 |
| ·The protocols of siRNA tranfection | 第20-21页 |
| ·RNA extraction and reverse transcriptase PCR(RT-PCR) | 第21-24页 |
| ·The protocol of total RNA extraction | 第21-22页 |
| ·RT-PCR | 第22-24页 |
| ·Agarose eletrophoresis | 第24页 |
| ·SDS-PAGE electrophoresis and western blot | 第24-25页 |
| ·Analysis of apoptosis by FACS(Fluorescence-Activated Cell Sorter) | 第25页 |
| ·Double staining | 第25页 |
| ·Propidium iodide staining | 第25页 |
| ·Cell viability assay (or WST assay) | 第25页 |
| ·Statistical analysis | 第25-26页 |
| Chapter 3.GD1a induces FBJ-cell apoptosis under serum-free conditions | 第26-32页 |
| ·GD1a is responsible for cell death in FBJ cells | 第26-29页 |
| ·FBJ-S1 cells undergo apoptosis under serum starvation | 第29-30页 |
| ·FBJ cells undergo apoptosis due to neither ceramide or GD3 | 第30-32页 |
| Chapter 4.Caveolin-1 is responsible for FBJ-cell apoptosis under serum-free conditions | 第32-35页 |
| ·Caveolin-1 expression is responsible for FBJ-cell apoptosis | 第32-33页 |
| ·Survivin is related to FBJ-cell apoptosis induced by GD1a | 第33-35页 |
| Chapter 5.The mechanisms of GD1a-induced FBJ-cell apoptosis | 第35-41页 |
| ·FBJ-LL cell-conditioned medium helps FBJ-S1 cells to survive in serum-free medium | 第35-37页 |
| ·Inhibition of p38 MAPK reverses the sensitivity of FBJ-S1 cells to apoptosis | 第37-38页 |
| ·Inhibitors of endocytosis promote FBJ-S1 cells survival | 第38-39页 |
| ·Cyclosporin A makes FBJ-S1 cells to survive under serum starvation | 第39-41页 |
| Chapter 6.Exogenous gangliosides induce FBJ-LL cell death under serum-free conditions | 第41-43页 |
| ·Exogenous gangliosides induce FBJ-LL cell death under serum-free conditions | 第41-43页 |
| Chapter 7.Conclusions and discussion | 第43-45页 |
| References | 第45-49页 |
| Published paper | 第49-50页 |
| Acknowledgements | 第50页 |
