| Abstract | 第1-7页 |
| 摘要 | 第7-11页 |
| Chapter 1 General Introduction | 第11-33页 |
| ·Fundamental reasons for studying oil hydrocarbon-associated microbial communities | 第11-12页 |
| ·Anaerobic Degradation of Petroleum Hydrocarbons | 第12-31页 |
| ·Aromatic Hydrocarbons | 第13-14页 |
| ·Saturated hydrocarbons | 第14-15页 |
| ·Microbial communities involved in anaerobic degradation of alkanes | 第15-27页 |
| ·Biochemical activation strategies for anaerobic degradation of alkanes | 第27-31页 |
| ·Summary | 第31-32页 |
| ·Summary of experimental approach | 第32-33页 |
| Chapter 2 Limited diversity of anaerobes in production water from a hightemperature petroleum reservoir | 第33-47页 |
| ·Site and sampling | 第33页 |
| ·Materials and Methods | 第33-40页 |
| ·Equipments | 第33-34页 |
| ·Reagents and culture media used for molecular cloning | 第34-35页 |
| ·Chemical analyses | 第35-36页 |
| ·Nucleic acid extraction | 第36页 |
| ·PCR amplifications 16S rRNA genes | 第36-37页 |
| ·PCR amplification of bssA and bamA gene fragments | 第37-38页 |
| ·Construction of clones libraries | 第38-39页 |
| ·Sequencing and phylogenetic analysis | 第39-40页 |
| ·Results and Discussion | 第40-46页 |
| ·Physicochemical characteristics of the production water from Well 3-18-35 | 第40页 |
| ·Phylogenetic analyses of microbial assemblage in the production water | 第40-46页 |
| ·Summary | 第46-47页 |
| Chapter 3 Methanogenic alkane-dependent microbial community derived fromproduction water of high temperature petroleum reservoir | 第47-62页 |
| ·Site and sampling | 第47页 |
| ·Materials and Methods | 第47-51页 |
| ·Preparation of enrichment cultures | 第47-48页 |
| ·Chemical analyses | 第48-49页 |
| ·Nucleic acid extraction | 第49页 |
| ·PCR amplifications 16S rRNA genes | 第49-50页 |
| ·PCR amplification of alkylsuccinate synthase α-subunit (assA) and methyl-coenzymeM reductase subunit A (mcrA) genes fragments | 第50页 |
| ·Construction of clones libraries | 第50-51页 |
| ·Sequencing and phylogenetic analysis | 第51页 |
| ·Results and Discussion | 第51-60页 |
| ·Physicochemical characteristics of the production water from SL34 | 第51页 |
| ·Alkane-dependent methanogenesis in thermophilic enrichment cultures | 第51-52页 |
| ·Microbial community composition of the thermophilic n-alkane-dependentmethanogenic cultures | 第52-60页 |
| ·Summary | 第60-62页 |
| Chapter 4 Anaerobic degradation of crude oil by a thermophilic methanogenicconsortium | 第62-74页 |
| ·Materials and methods | 第62-64页 |
| ·Transfer of primary enrichment and preparation of methanogenic oil-degradingcultures | 第62-63页 |
| ·Crude oil and metabolites analyses | 第63-64页 |
| ·Nucleic acid extraction | 第64页 |
| ·PCR amplifications of bacterial and archaeal 16S rRNA genes | 第64页 |
| ·Construction of clones' libraries | 第64页 |
| ·Sequencing and phylogenetic analysis | 第64页 |
| ·Results and Discussion | 第64-71页 |
| ·Crude oil degradation and methane formation in thermophilic enrichment cultures | 第64-67页 |
| ·Analysis of volatile fatty acids (VFA) in the methanogenic enrichment cultures | 第67-68页 |
| ·Microbial community composition of the oil-degrading methanogenic cultures | 第68-71页 |
| ·Summary | 第71-74页 |
| Chapter 5 Bacterial diversity in methanogenic alkane-degrading enrichmentestablished from oily sludge characterized by catabolic functional genes | 第74-81页 |
| ·Materials and Methods | 第75-76页 |
| ·Sampling site of oily sludge | 第75页 |
| ·Preparation of enrichment cultures | 第75页 |
| ·Nucleic acid extraction | 第75页 |
| ·PCR amplification of functional gene fragments | 第75页 |
| ·Sequencing and phylogenetic analysis | 第75页 |
| ·Construction of catabolic functional genes clones libraries | 第75-76页 |
| ·Results and Discussion | 第76-79页 |
| ·Summary | 第79-81页 |
| Chapter 6 General Conclusions and Future Work | 第81-84页 |
| References | 第84-98页 |
| Acknowledgements | 第98-99页 |
| Publications List | 第99-100页 |
| Appendix | 第100-102页 |
