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三种稻飞虱(灰飞虱、裼飞虱、白背飞虱)对温度胁追耐受性的比较

Abstract第6-7页
摘要第8-18页
CHAPTER 1 Literature Review第18-43页
    1.1 Rice- Introduction and production第18-19页
    1.2 Global Rice consumption from 2008-09 to 2017-18第19页
    1.3 Insect pests of rice crop第19-21页
        1.3.1 Rice planthoppers第20页
        1.3.2 Rice leaffolders第20-21页
        1.3.3 Rice stem borers第21页
    1.4 Rice Planthoppers第21-27页
        1.4.1 Planthoppers biology第23页
        1.4.2 Significance and threat of rice planthoppers第23-26页
        1.4.3 Planthopper damage to rice crop第26-27页
    1.5 Global warming and climate change impact on insect pests第27-30页
    1.6 Thermoregulation in insect pests第30-32页
    1.7 Geographical changes in heat tolerance第32页
    1.8 Thermal tolerance indices第32-35页
        1.8.1 Critical thermal temperatures第33页
        1.8.2 Heat coma temperatures第33-34页
        1.8.3 Lethal temperatures第34页
        1.8.4 Seasonal and rapid cold hardening第34-35页
    1.9 Thermal indices effect on insect pests including planthoppers第35-37页
    1.10 Survival approaches of insects at high temperatures第37-38页
    1.11 Heat shock proteins and their functions第38-42页
        1.11.1 Heat shock protein analysis limitations in insects第40-41页
        1.11.2 Hardening effects in insects第41-42页
    1.12 Objectives第42-43页
CHAPTER 2 Comparison of upper sublethal and lethal temperatures in three rice planthopper species第43-54页
    2.1 Introduction第43-44页
    2.2 Material and Methods第44-47页
        2.2.1 Insect cultures第44-45页
        2.2.2 Determination of critical thermal maximum (CTmax)第45页
        2.2.3 Determination of heat coma temperature (HCT)第45-46页
        2.2.4 Determination of upper lethal temperature (ULT)第46页
        2.2.5 Data analysis第46-47页
    2.3 Results第47-51页
        2.3.1 CTmax第47-49页
        2.3.2 HCT第49-50页
        2.3.3 ULT第50-51页
    2.4 Discussion第51-54页
CHAPTER 3 Induced expression levels of Hsp90, Hsp70 and Hsp60 in three rice planthopper species in response to thermal stress treatments第54-68页
    3.1 Introduction第54-55页
    3.2 Material and Methods第55-60页
        3.2.1 Insect cultures第55-56页
        3.2.2 Thermal stress treatments for analysis of HSPs第56页
        3.2.3 Planthopper survival under different thermal stress treatments第56页
        3.2.4 Isolation of RNA第56-57页
        3.2.5 Synthesis of first-strand c DNA第57页
        3.2.6 PCR第57-58页
        3.2.7 Sequences of c DNA第58页
        3.2.8 q RT-PCR Detection第58-59页
        3.2.9 Data analysis第59-60页
    3.3 Results第60-64页
        3.3.1 Survival rate of three planthopper species under four different temperatures第60-61页
        3.3.2 Expression profiles of three heat shock protein genes第61-64页
            3.3.2.1 Expression profiles of Hsp90第61-62页
            3.3.2.2 Expression profiles of Hsp70第62-63页
            3.3.2.3 Expression profiles of Hsp60第63-64页
    3.4 Discussion第64-68页
CHAPTER 4 General Discussion and Conclusion第68-72页
CONCLUSION第72-73页
REFERENCES第73-98页
ACKNOWLEDGEMENTS第98-100页
Author biography第100-104页

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