| 摘要 | 第4-5页 |
| Abstract | 第5页 |
| List of abbreviations | 第9-11页 |
| Chapter 1 Introduction | 第11-14页 |
| 1.1 Waves of myogenesis in animal | 第11-12页 |
| 1.2 Factors affecting animal muscle development | 第12-13页 |
| 1.3 Application of microarray technology in skeletal muscle development research | 第13页 |
| 1.4 Summary | 第13-14页 |
| Chapter 2 Morphology and gene expression in LD muscle of ovine fetus | 第14-32页 |
| 2.1 Introduction | 第14页 |
| 2.2 Objective for current research | 第14-15页 |
| 2.3 Materials and Methods | 第15-19页 |
| 2.3.1 Ethics statement | 第15页 |
| 2.3.2 Animals | 第15页 |
| 2.3.3 Samples collection and RNA extraction | 第15-16页 |
| 2.3.4 Histology analysis | 第16页 |
| 2.3.5 Analysis for Gene expression data | 第16-18页 |
| 2.3.5.1 Microarray transcript profiling | 第16-17页 |
| 2.3.5.2 Statistical analysis of microarray data | 第17页 |
| 2.3.5.3 Biological interpretation of the differentially expressed genes | 第17-18页 |
| 2.3.5.4 Visual exploration of the biological interaction network | 第18页 |
| 2.3.5.5 Identification of conserved cis-regulatory motifs | 第18页 |
| 2.3.6 Quantitative real-time reverse transcription PCR | 第18-19页 |
| 2.4 Results | 第19-28页 |
| 2.4.1 Changes in the myofiber traits in LD muscle | 第19-20页 |
| 2.4.2 Global gene expression in LD throughout investigation | 第20-21页 |
| 2.4.3 Clusters of the differentially expressed genes | 第21-24页 |
| 2.4.4 Identification of significantly over-represented GO terms,keywords,and pathways in each cluster | 第24-25页 |
| 2.4.5 Module visualization and differential interaction networks analysis | 第25-27页 |
| 2.4.6 Identification of conserved cis-regulatory motifs in gene groups | 第27-28页 |
| 2.5 Discussion | 第28-32页 |
| 2.5.1 Changes in muscle fiber number and size during the last half of ovine gestation | 第28-29页 |
| 2.5.2 Oxidative metabolism in muscles during the last third of ovine gestation | 第29-30页 |
| 2.5.3 Lipid metabolism in muscles during the last third of ovine gestation | 第30-31页 |
| 2.5.4 Mechanisms controlling the changes in gene expression during the second half of ovine gestation | 第31-32页 |
| 2.6 Conclusions | 第32页 |
| References | 第32-41页 |
| ACKNOWLEDGMENTS | 第41-42页 |
| Supporting Information | 第42-75页 |
| PUBLICATION | 第75-76页 |
