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Identification of Favorable Alleles of ZmNYC1 Gene for Chlorophyll Related Traits by Association Mapping in Maize (Zea Mays L.)

ABSTRACT第5-6页
Chapter Ⅰ INTRODUCTION第9-21页
    1.1 The Important of Maize第9-10页
        1.1.1 Maize Production in the World第9页
        1.1.2 Maize Genomics第9-10页
    1.2 Chlorophyll第10-16页
        1.2.1 Characteristic of Chorophyll第10-11页
        1.2.2 Chlorophyll Metabolisms第11-14页
        1.2.3 Genes Involved in Chlorophyll Metabolisms第14-15页
        1.2.4 Role of Non-Yellow Coloring 1(NYC1)Gene in Chlorophyll Metabolisms第15-16页
    1.3 Genetic Dissection of Quantitative Traits第16-19页
        1.3.1 Linkage Mapping第16-17页
        1.3.2 Association Mapping第17-18页
        1.3.3 Advantages of Association Mapping over Linkage Mapping第18-19页
    1.4 Objectives第19-20页
    1.5 Research Route第20-21页
Chapter Ⅱ MATERIALS AND METHODS第21-26页
    2.1 Plant Materials and Experimental Design第21页
        2.1.1 Laboratory plant第21页
        2.1.2 Field plant第21页
    2.2 Chlorophyll Determination第21-23页
        2.2.1 SPAD readings第21-22页
        2.2.2 Chlorophyll Compound Determination第22-23页
    2.3 Characterization of ZmNYC1 sequence第23-25页
        2.3.1 ZmNYC1 cloned in silico第23页
        2.3.2 ZmNYC1 re-sequencing第23-25页
    2.4 Association Analysis of ZmNYC1第25页
    2.5 Gene Expression Analysis for Associated Sites第25-26页
Chapter Ⅲ RESULTS第26-40页
    3.1 Phenotypic Variation in Association Panel第26-32页
        3.1.1 Chlorophyll Content Variation at Seedling Stage第26-27页
        3.1.2 Difference in Chlorophyll Concentration under Light and Dark第27-28页
        3.1.3 Chlorophyll Content Variation at Mature Stage第28-30页
        3.1.4 Correlation Coefficients among the Chlorophyll Related Traits第30-32页
    3.2 Genotype Diversity in Association Panel第32-34页
    3.3 Association Results第34-39页
        3.3.1 Favorable Sites of ZmNYC1 gene at Seedling Stage第34-37页
        3.3.2 Favorable Sites of ZmNYC1 gene in Sensitive or Insensitive to Dark Inbreds at Seedling Stage第37-38页
        3.3.3 Favorable Sites of ZmNYC1 gene at Mature Stage第38-39页
    3.4 Expression Pattern of ZmNYC1第39-40页
Chapter Ⅳ DISCUSSIONS第40-43页
    4.1 Chlorophyll Content Variation at Seedling Stage and at Mature Stage第40页
    4.2 Differential Response to Dark Treatment in Maize Inbred Lines第40-41页
    4.3 Favorable Sites and Expression Pattern of ZmNYC1 gene第41-43页
Chapter Ⅴ OVERALL CONCLUSIONS AND SUGGESTIONS第43-44页
SUPPLEMENTARY DATA第44-62页
REFERENCES第62-72页
APPENDICES第72-79页
LIST OF ABBREVIATIONS第79-80页
ACKNOWLEDGEMENTS第80-82页
BIOGRAPHICAL SKETCH第82页

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