| Abstract in Chinese | 第1-9页 |
| Abstract in English | 第9-11页 |
| 1 Literature review | 第11-28页 |
| ·Mammary development and lactation mechanism | 第11-13页 |
| ·Mammary gland development process | 第11-12页 |
| ·Lactation mechanism | 第12-13页 |
| ·Lactation signal transduction pathways | 第13-17页 |
| ·Milk protein synthesis and signal transduction | 第13-15页 |
| ·Regulation of amino acids for lactation | 第15-17页 |
| ·Research progress of 1433 | 第17-25页 |
| ·Introduction of 1433 family | 第17页 |
| ·Structure and function of 1433 proteins | 第17-19页 |
| ·1433 regulatory proteins | 第19-20页 |
| ·Regulation of 1433 by phosphorylation | 第20-22页 |
| ·Role of 1433 proteins in regulating cell cycle | 第22-23页 |
| ·Role of 1433 in apoptosis | 第23-25页 |
| ·Bioinformatics of 1433γ | 第25-26页 |
| ·Protein sequence databases | 第25-26页 |
| ·Structural databases | 第26页 |
| ·Protein-protein interactions | 第26页 |
| ·The purpose and significance of research | 第26-28页 |
| 2 Materials and methods | 第28-46页 |
| ·Reagents and Instruments | 第28-29页 |
| ·The main laboratory reagents | 第28页 |
| ·The main experimental apparatus | 第28-29页 |
| ·Methods | 第29-44页 |
| ·Primary culture and identification of dairy mammary epithelial cells | 第29-30页 |
| ·Effects of amino acid and hormone on expression of 1433γ and lactogenes- is in DCMECs | 第30-39页 |
| ·Functional analysis of 1433γ | 第39-44页 |
| ·Data processing | 第44页 |
| ·Bioinformatics | 第44-46页 |
| ·Protein structure | 第44页 |
| ·Secondary structure | 第44页 |
| ·Domains | 第44-45页 |
| ·Post-translational modifications | 第45页 |
| ·Sub-cellular localization | 第45页 |
| ·Phylogeny of the 1433γ | 第45-46页 |
| 3 Results and Analysis | 第46-69页 |
| ·Primary cultures and identification of dairy mammary | 第46-48页 |
| ·Effects of amino acids and hormones on expression of 1433γ and lactogenesis in DCMECs | 第48-50页 |
| ·Effects of Methionine on expression of 1433γ | 第48页 |
| ·Effects of amino acid and hormone on lactogenesis | 第48-50页 |
| ·Confocal analysis of the expression of 1433γ | 第50页 |
| ·1433γ gene overexpression | 第50-57页 |
| ·Construction of eukaryotic expression vector p GCMV-IRES -EGFP143-3γ | 第50-52页 |
| ·Cell Transfection | 第52页 |
| ·Eukaryotic expression vector p GCMV-IRES-EGFP143- 3γ transient transfection | 第52-55页 |
| ·Eukaryotic expression vector p GCMV-IRES-EGFP143-3γ stably transfected | 第55-57页 |
| ·1433γ gene silence | 第57-59页 |
| ·Mechanism of 1433γ regulating lactogenesis in DCMEC via the m TOR/S6K1 signaling pathway | 第59-60页 |
| ·Results of bioinformatics | 第60-69页 |
| ·Similar protein of 1433γ, domains, post- translational modifications and motifs | 第60-63页 |
| ·Secondary structure | 第63-65页 |
| ·Phylogeny of the 1433γ | 第65-66页 |
| ·Subcellular location prediction of 1433γ | 第66页 |
| ·Gene ontology analysis of 1433γ proteins | 第66-68页 |
| ·Molecular weight and isoelectric point of 1433γ | 第68-69页 |
| 4 Discussion | 第69-75页 |
| ·Dairy mammary epithelial cell culture, purification and | 第69页 |
| ·Analysis of 1433γ protein localization within the cell | 第69-70页 |
| ·Analysis Overexpression of 1433-γ gene fragment in DCMECs | 第70-71页 |
| ·Impact of 1433-γ lactating mammary epithelial cells of cows | 第71-72页 |
| ·Bioinformatics of 1433γ | 第72-75页 |
| 5 Conclusion | 第75-76页 |
| Acknowledgements | 第76-77页 |
| References | 第77-88页 |
| Appendix List of abbreviations | 第88-90页 |
| Paper published in the period of Ph.D. education | 第90-91页 |
