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Isolation, Characterization And Identification of Probiotic Bacteria from Chinese Soft-shell Turtle(Trionyx Sinensis)

1. Introduction第8-13页
    1.1 Biology of the Soft-shell Turtle第8-9页
    1.2. Economic Importance of the Soft-shell Turtle第9-10页
    1.3. Challenges and Diseases on the Culture of Soft-Shell Turtle第10-11页
    1.4. Definition of Probiotic第11-12页
    1.5. 16S r RNA PCR第12-13页
    1.6. Objectives of the Study第13页
2. Literature Review第13-19页
    2.1. Benefits of Probiotics in Aquaculture第13-14页
    2.2. Microorganisms used as Probiotics in Aquaculture第14-17页
    2.3. Selection Criteria for Potential Probiotic Bacteria第17-18页
    2.4. Probiotic Bacteria in Soft-shell Turtle第18-19页
3. Materials and Methods第19-27页
    3.1. Potential probiotic bacteria isolation and screening from T.sinensis第19-22页
        3.1.1. Isolation for potential probiotic bacteria strains第19-20页
        3.1.2. Purification of isolated potential probiotic bacteria strains第20页
        3.1.3. Indicate bacteria selection第20页
        3.1.4. Commercial probiotic bacteria and antibiotic selection第20-21页
        3.1.5. In vitro indicate bacteria inhibition tests第21-22页
        3.1.6. In vitro supernatant inhibition tests第22页
    3.2. Results第22-26页
        3.2.1. In vitro indicate bacteria inhibition tests第22-24页
        3.2.2. In vitro supernatant inhibition tests第24-26页
    3.3. Discussion第26-27页
4. Identification of the potential bacteria strains isolated from T. sinensis第27-31页
    4.1. Materials and Methods第27-28页
        4.1.1.16s r RNA PCR Method and Sequencing第27页
        4.1.2. Gel Electrophoresis第27-28页
    4.2. Results第28-30页
    4.3. Discussion第30-31页
5. Spectrophotometer determination of bacteria and supernatant's protein concentrations第31-34页
    5.1. Materials and Methods第31-32页
        5.1.1. Bacteria Concentrations第31页
        5.1.2. Protein Concentrations第31-32页
    5.2. Results第32-33页
    5.3. Discussion第33-34页
6. Conclusion第34-35页
7. References第35-38页
Acknowledgements第38-39页
Abstract第39页
中文摘要第41页

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