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水稻种质资源中抗旱基因SNAC1的表达多样性

Chinese Abstract第7-9页
English Abstract第9-10页
Abbreviations第11-12页
1 Introduction第12-14页
    1.1 Research purposes and significance第12-13页
    1.2 Originality and creativeness第13-14页
2 Literature review第14-36页
    2.1 Rice cultivation ecosystems and their hydrological status第14-16页
    2.2 Drought and its influence on rice production第16-19页
        2.2.1 Physiological effect and economic impacts第17-18页
        2.2.2 Types of drought stress第18-19页
    2.3 Drought resistance improvement by marker assisted selection第19-27页
        2.3.1 Identification of drought-related QTL第20-23页
        2.3.2 Marker-assisted selection to improve drought resistance第23-26页
        2.3.3 Molecular markers for drought resistance第26-27页
    2.4 Diversity of drought resistance-related genes in rice第27-29页
    2.5 Rice breeding for drought tolerance第29-34页
        2.5.1 Conventional breeding第30-31页
        2.5.2 The advance breeding第31-33页
        2.5.3 Participatory breeding第33-34页
    2.6 Screening for drought resistance第34-36页
3 Materials and Methods第36-40页
    3.1 Plants,materials,and growth condition第36页
    3.2 Stress treatment and sampling第36-37页
    3.3 RNA isolation and reverse transcription第37页
    3.4 Quantitative real-time polymerase chain reaction(qRT-PCR)analysis第37-38页
    3.5 Association analysis and haplotype analysis第38-40页
4 Results第40-48页
    4.1 Variations of SNAC1 expression in rice germplasms第40-42页
    4.2 Genetic variation and linkage disequilibrium at the SNAC1 locus第42-43页
    4.3 Population structure and association analysis of SNAC1第43-44页
    4.4 Haplotype analysis of SNAC1第44-48页
5 Discussion第48-52页
    5.1 Expression level polymorphisms of SNAC1第48-49页
    5.2 Population structure and association analysis of SNAC1第49页
    5.3 Haplotype analysis of SNAC1第49-52页
References第52-65页
Appendix第65-77页
    1 Protocols第65-68页
        1.1 TRIzol reagent RNA isolation method第65页
        1.2 Reverse transcription reaction polymerase chain reaction(RT-PCR)第65-67页
        1.3 Quantitative Real Time Polymerase Chain Reaction(qRT-PCR)第67-68页
    2 Supplemental data第68-77页
        Supplementary Table 1第68-72页
        Supplementary Table 2第72-74页
        Supplementary Table 3第74-76页
        Supplementary Table 4第76-77页
Resume第77-83页
Acknowledgement第83页

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