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几种药用芳香植物的抗氧化、抗菌、降糖和抗癌活性

Dedication第6-7页
Acknowledgements第7-12页
List of Abbreviations第12-14页
Abstract第14-16页
摘要第17-28页
CHAPTER 1 General Introduction and Literature Review第28-54页
    1.1 Introduction第28页
    1.2 Aromatic Medicinal Plants from Yemen第28-31页
        1.2.1 Asteraceae第28-30页
        1.2.2 Lamiaceae第30-31页
    1.3 Antioxidant Natural Plant第31-36页
        1.3.1 Phenolic Compounds第32-33页
        1.3.2 Flavonoids第33-34页
        1.3.3 Tannins第34-35页
        1.3.4 Anthocyanins第35-36页
    1.4 Essential oil of plants第36-37页
    1.5 Antioxidants activity第37-40页
        1.5.1 Antioxidant activity using (DPPH) radical scavenging第38页
        1.5.2 Antioxidant activity using β-Carotene bleaching assay第38-39页
        1.5.3 Antioxidant activity using Reducing power第39页
        1.5.4 Tocopherol and their antioxidant activity and mineral contents第39-40页
    1.6 Macroporous resin第40页
    1.7 Fish spoilage and foodborne pathogenic bacteria第40-43页
        1.7.1 Listeria monocetogenes第41页
        1.7.2 Escherichia coli第41-42页
        1.7.3 Staphelococcus aureus第42-43页
    1.8 Additives used in fish preservation第43-44页
    1.9 Antioxidant Health第44页
    1.10 Diabetes mellitus第44-45页
    1.11 Breast Cancer第45-46页
        1.11.1 What is breast cancer?第45页
        1.11.2 The normal breast in women第45-46页
        1.11.3 Breast cancer MCF-7第46页
    1.12 Aim of the study第46-47页
        1.12.1 Main objective第46页
        1.12.2 The Specific objectives of this study were第46-47页
    1.13 References第47-54页
CHAPTER 2 Antimicrobial and Antioxidant Activities of the Essential Oils of Some AromaticMedicinal Plants (Pulicaria inuloides-Asteraceae and Ocimum forskolei-Lamiaceae)第54-66页
    2.1 Introduction第54页
    2.2 Material and Methods第54-56页
        2.2.1 Plant collection and identification第54-55页
        2.2.2 Isolation of essential oil第55页
        2.2.3 Identification of essential oil第55页
        2.2.4 Determination of total phenolic content第55-56页
        2.2.5 Determination of radical scavenging activity第56页
        2.2.6 β-carotene bleaching assay (BCB)第56页
    2.3 Antimicrobial activity第56-57页
        2.3.1 Microorganisms第56-57页
        2.3.2 Disc diffusion assay第57页
        2.3.3 Determination of minimal inhibitory concentration (MIC)第57页
        2.3.4 Statistical analysis第57页
    2.4 Results and discussion第57-63页
        2.4.1 Chemical composition of the essential oils第57-58页
        2.4.2 Total phenolic content and antioxidant activity第58-59页
        2.4.3 Antimicrobial activity第59-63页
    2.5 Conclusion第63页
    2.6 References第63-66页
CHAPTER 3 Total Phenolic Content and Antioxidant, Antimicrobial Activity of Some Yemeni Plants(Roots)第66-79页
    3.1 Introduction第66-67页
    3.2 Materials and methods第67-70页
        3.2.1 Essential oils isolation第67-68页
        3.2.2 GC-MS analysis第68页
        3.2.3 Determination of total phenolic content第68页
        3.2.4 Determination of DPPH radical scavenging activity第68页
        3.2.5 Bleaching of β-carotene assay (BBC)第68-69页
        3.2.6 Assessment of antimicrobial activity第69页
        3.2.7 Statistical analysis第69-70页
    3.3 Results and discussion第70-75页
        3.3.1 Chemical composition of the essential oils第70-72页
        3.3.2 Total phenolic content and antioxidant activity第72-74页
        3.3.3 Antimicrobial activity第74-75页
    3.4 Conclusion第75页
    3.5 References第75-79页
CHAPTER 4 Comparative Study of various methods for extraction of Antioxidant,Antibacterialcompounds from Plant (Pulicaria inuloides-Asteraceae)第79-90页
    4.1 Introduction第79页
    4.2 Materials and Methods第79-82页
        4.2.1 Experimental Plant第79-80页
        4.2.2 Preparation of the Extracts第80页
        4.2.3 Determination of Total Phenolic Contents (TPC)第80页
        4.2.4 Determination of Total Flavonoid Contents (TFC)第80页
        4.2.5 Determination of Tannins第80页
        4.2.6 Determination of Total Anthocyanin Content第80-81页
        4.2.7 Determination of DPPH Radical Scavenging Activity第81页
        4.2.8 β-Carotene Bleaching Test第81页
        4.2.9 Metal Chelating Activity第81页
        4.2.10 Antimicrobial activity第81页
        4.2.11 Statistical Analysis第81-82页
    4.3 Results and discussion第82-87页
        4.3.1 Total Phenolic, Total Flavonoid,Total Anthocyanins and Total Tannin Contents Activity第82-83页
        4.3.2 DPPH Radical- Scavenging Activity第83-84页
        4.3.3 Metal Chelating Activity (MCA)第84-85页
        4.3.4 β-Carotene linoleate Bleaching Test第85-86页
        4.3.5 Antimicrobial activity第86-87页
    4.4 Conclusion第87页
    4.5 References第87-90页
CHAPTER 5 Bioactivity of the Phenolic constituents of Pulicaria inuloides and its antibacterial and antioxidant activities第90-114页
    5.1 Introduction第90-91页
    5.2 Materials and Methods第91-96页
        5.2.1 Plant collection, identification and documentation第91页
        5.2.2 Chemicals and Machine第91页
        5.2.3 Determination of tocopherol composition第91-92页
        5.2.4 Determination of phenolic compounds by macroporous resin第92页
        5.2.5 GC-MS analysis and Identification and Quantification of Phenolic CompoundsUsing High-performance Liquid Chromatography (HPLC)第92-93页
        5.2.6 Standards preparation第93页
        5.2.7 Antioxidant assay第93-94页
        5.2.8 Antimicrobial assay第94-95页
        5.2.9 Minerals content第95页
        5.2.10 Scanning electron microscopy (SEM)第95页
        5.2.11 Sample preparation for Transmission electron microscope (TEM-JEM-2100)第95页
        5.2.12 Statistical analysis第95-96页
    5.3 Results and Discussion第96-106页
        5.3.1 Chemical Composition of vitamins第96-97页
        5.3.2 Chemical Composition of the phenolic compounds第97-99页
        5.3.3 Bioactive compounds第99-102页
        5.3.4 Antioxidant activity第102页
        5.3.5 These results were lower than what had previously been reported第102-106页
    5.4 Mineral determination第106页
    5.5 Scanning electron microscopy (SEM)第106-108页
        5.5.1 Transmission electron microscopy (TEM)第107页
        5.5.2 Energy-dispersive X-ray spectroscopy (EDX)第107-108页
    5.6 Conclusion第108-109页
    5.7 References第109-114页
CHAPTER 6 Antibacterial activities of the essential oils of some aromatic medicinal plants to controlpathogenic bacteria and extend the shelf-life of China seafood第114-130页
    6.1 Introduction第114-115页
    6.2 Materials and methods第115-119页
        6.2.1 Plant material and essential oil第115页
        6.2.2 Preparation and maintenance of bacteria第115-116页
        6.2.3 Fish preparation第116页
        6.2.4 Treatment preparation: fillet fish第116-117页
        6.2.5 Fish inoculation and treatment application第117-118页
        6.2.6 Enumeration of Listeria monocytogenes第118页
        6.2.7 Enumeration of Escherichia coli第118页
        6.2.8 Enumeration of Staphylococcus aureus第118页
        6.2.9 Enumeration of Standard Plate Count (SPC)第118页
        6.2.10 Determination of pH第118-119页
        6.2.11 Sensory evaluation第119页
        6.2.12 Statistical analysis第119页
    6.3 Results and discussion第119-125页
        6.3.1 Microbiological composition of samples第119-120页
        6.3.2 Antimicrobial activity in fish fillets第120-122页
        6.3.3 Determination of pH第122-123页
        6.3.4 Sensory Evaluation第123-125页
    6.4 Conclusions第125页
    6.5 References第125-130页
CHAPTER 7 In Vitro and in Vivo evaluation of Antidiabetic Activity of Pulicaria inuloides-Asteraceae第130-148页
    7.1 Introduction第130-131页
    7.2 Materials and methods第131-136页
        7.2.1 Chemicals第131页
        7.2.2 Collection and Identification of Plant Material第131页
        7.2.3 Isolation of essential oil第131页
        7.2.4 Phytochemical screening第131-133页
        7.2.5 Alpha-amylase inhibition assay第133页
        7.2.6 α-Glucosidase inhibitory assay第133-134页
        7.2.7 In-vivo evaluation of antidiabetic activity of essential oil of plant第134-135页
        7.2.8 Biochemical analysis第135页
        7.2.9 Tissue preparation for histology第135-136页
        7.2.10 Statistical Analysis第136页
    7.3 Results and discussion第136-144页
        7.3.1 Phytochemical Analysis第136-137页
        7.3.2 Inhibition assay for α- amylase and α- glucosidase第137-138页
        7.3.3 In vivo antidiabete activity of Pulicaria inuloides leaf essential oil第138-144页
    7.4 Conclusion第144页
    7.5 References第144-148页
CHAPTER 8 Anticancer, Antimicrobial and Antioxidant Activities of the Essential Oils of SomeAromatic Medicinal Plants (Pulicaria inuloides-Asteraceae)第148-160页
    8.1 Introduction第148-149页
    8.2 Materials and Methods第149页
        8.2.1 Plant material第149页
        8.2.2 Isolation of the essential oil第149页
    8.3 Chemicals第149页
    8.4 Anti-pathogenic bacterial activity第149-152页
        8.4.1 Pathogenic bacterial strains第149-150页
        8.4.2 Agar disk diffusion (The agar well diffusion method)第150页
        8.4.3 Determination of minimal inhibitory concentration (MIC)第150页
        8.4.4 Free radical-scavenging effect of PiEO第150-151页
        8.4.5 Cancer Cell Lines第151页
        8.4.6 Cell culture media RPMI 1640 proportions第151页
        8.4.7 Cell subculture第151页
        8.4.8 Cytotoxicity assay第151-152页
        8.4.9 Morphological examination第152页
        8.4.10 Statistical analysis第152页
    8.5 Results and Discussion第152-158页
        8.5.1 Antimicrobial activity of PiEO第152-153页
        8.5.2 Free radical scavenging effect第153-154页
        8.5.3 Cytotoxic activity of PiEO on MCF-7 cells第154-155页
        8.5.4 Morphological analysis第155-158页
    8.6 Conclusion第158页
    8.7 References第158-160页
General Conclusions and Recommendations第160-163页
    General conclusions第160-161页
    Key innovation of the thesis第161页
    Recommendations第161-163页
List of Publications第163页

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